Additional File 3.

The resulting image of an hESC colony cultured in a conventional hESC medium. (a) The hESC colony was analyzed using the defined area analysis protocol. The resulting image of the analyzed colony is shown in (b). Green color represents undifferentiated areas and blue represents differentiated areas according to the analysis result. (c) The hESC colony was immunohistochemically characterized using an antibody against an undifferentiated hESC marker, Nanog (green), and a differentiated hESC marker, SSEA1 (red). Scale bar = 200 μm.

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Narkilahti et al. BioMedical Engineering OnLine 2007 6:11   doi:10.1186/1475-925X-6-11