|
2PE excitation parameters. |
|||
| FLUOROPHORES |
λ (nm) |
ηδ2 |
δ2 |
|
|
|||
| Extrinsic fluorophores |
|||
| Bis-MSB |
691/700 |
6.0 ± 1.8 |
6.3 ± 1.8 |
| Bodipy |
920 |
17 ± 4.9 |
- |
| Calcium Green |
740–990 |
- |
~80 |
| Calcofluor |
780/820 |
- |
- |
| Cascade blue |
750–800 |
2.1 ± 0.6 |
~3 |
| Coumarin 307 |
776, 700–800 |
19 ± 5.5 |
~20 |
| CY2 |
780/800 |
- |
- |
| CY3 |
780 |
- |
- |
| CY5 |
780/820 |
- |
- |
| DAPI (free) |
700/720 |
0.16 ± 0.05 |
~3.5 * |
| Dansyl |
700 |
1 |
- |
| Dansyl Hydrazine |
700 |
0.72 ± 0.2 |
- |
| Dil |
700 |
95 ± 28 |
- |
| Filipin |
720 |
- |
- |
| FITC |
740–820 |
- |
~25–38 * |
| Fluorescein (pH ~11) |
780 |
- |
38 ± 9.7 |
| Fura-2 (free) |
700 |
11 |
- |
| Fura-2 (high Ca) |
700 |
12 |
- |
| Hoechst |
780/820 |
- |
- |
| Indo-1 (free) |
700 |
4.5 ± 1.3 |
12 ± 4 |
| Indo-1 (high Ca) |
590/700 |
1.2 ± 0.4 |
2.1 ± 0.6 |
| Lucifer Yellow |
840–860 |
0.95 ± 0.3 |
~2 |
| Nile Red |
810 |
- |
- |
| Oregon Green Bapta 1 |
800 |
- |
- |
| Rhodamine B |
840 |
- |
210 ± 55 |
| Rhodamine 123 |
780–860 |
- |
- |
| Syto 13 |
810 |
- |
- |
| Texas red |
780 |
- |
- |
| Triple probe (Dapi, FITC, and Rhodamine) |
720/740 |
- |
- |
| TRITC (rhodamine) |
800–840 |
- |
- |
Diaspro et al. BioMedical Engineering OnLine 2006 5:36 doi:10.1186/1475-925X-5-36 |
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